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Amjad Hameed, Razia Kuasar, and Arruje Hameed |
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· Preface
Gel electrophoresis has become a major technique in
modern molecular biology, with numerous applications. Agarose gel electrophoresis
is the mostly commonly used technique for DNA separations, and polyacrylamide
gel electrophoresis has revolutionized proteomics and genomics. Several basic
methods have been improved to the extent that researchers usually have to
proceed by assembling a protocol from many sources. In this book, the standard
protocols and numerous improvements are presented, and the various methods are
indexed and cross-referenced for ease of use. Each technique is introduced in a
way that assumes only a modicum of previous exposure. Readers will quickly gain
a clear perception of the mechanisms involved to help them practically apply
techniques and troubleshoot problems. Critical steps, potential errors, and
remedies are provided. Safety measures and environmental aspects are also
covered. The final part of the book contains detailed applications of
techniques in agriculture, diagnostics, diversity assessment, proteomics,
genomics, forensics, and biotechnology that will help readers in utilizing
these techniques in their respective disciplines.
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