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Amjad Hameed, Razia Kuasar, and Arruje Hameed |
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Illustrated: |
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· Contents
1. Historical view
2. Basics of electrophoresis
2.1.
Working with electrophoresis
2.2. Use
of buffers in electrophoresis
2.3. What are
gels?
2.4. What
are dyes?
2.4.1. Loading or tracking dyes
2.4.2. Marker or fluorescent dyes
2.5. What are standards?
2.6. Buffers and solutions preparation
3. Agarose gel electrophoresis
3.1.
Equipment and reagents
3.2. Preparing and running agarose
gels
3.3. Gel electrophoresis
3.4. Agarose gel analysis
3.5. Recent advances in technique
4. Polyacrylamide gel electrophoresis (PAGE)
4.1. Types of polyacrylamide gels
4.1.1.
Native PAGE
4.1.2.
Denaturing PAGE
4.2.
Continuous and discontinuous buffer systems
4.3.
Equipment and reagents
4.4. Preparing and running PAGE
4.5. Gel electrophoresis
4.6. Gel
analysis and molecular weight determination
4.7. Recent advances in technique
5. Isoelectric focusing (IEF)
5.1.
Concept behind technique
5.2.
Equipment and reagents
5.3.
Preparing and running
5.4. IEF analysis
5.5. Recent advances in technique
6. Two-dimensional PAGE (2-D PAGE)
6.1. Why and how 2-D
6.2.
Equipment and reagents
6.3.
Preparing and running 2-D gels
6.4.
2-D gel analysis
6.5.
Recent advances in technique
7. Applications
of gel electrophoresis
· About the Authors | · Preface
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