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Edited by Jiann-Tsyh Lin and Thomas A. McKeon |
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$99.75 |
April 2005 |
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Preface
High-performance
liquid chromatography (HPLC) is a powerful method for lipid analysis. This book
covers many practical and theoretical aspects of HPLC separation of acyl
lipids. A book with a similar title, High-Performance Liquid Chromatography
and Lipids: A Practical Guide, by William W. Christie, covering mainly acyl
lipids, was published in 1987. Since that time, a great number of papers
related to the HPLC of acyl lipids have been published, making an updated book
devoted exclusively to the HPLC of acyl lipids long overdue. Since lipids in
general are too broad to cover in one book, we have limited this book to fatty
acids and fatty acid棯ntaining lipids as acyl lipids, and have excluded other
lipids such as steroids, terpenoids, carotenoids and vitamin E (prenyl lipids).
This book's
18 chapters are aimed at the practical aspects of lipid separation. Since
practical and theoretical aspects of lipid separation are interrelated, one
chapter (Chapter 3) is devoted exclusively to the HPLC theory of acyl lipid
separation. HPLC of lipid derivatives is not emphasized in this book, except in
Chapter 1, covering fatty acid methyl esters; Chapter 2, devoted exclusively to
fatty acid derivatives; and Chapter 7, covering chiral phase HPLC. LC-MS is a
powerful tool for lipid analysis and has become more affordable and popular.
Therefore, two chapters focus on LC-MS using atmospheric pressure chemical
ionization (Chapter 10) and electrospray ionization (Chapter 11) for separation
and identification. In addition, one chapter is devoted to the LC-MS-MS of
bioactive lipids (Chapter 15), and many chapters also include some LC-MS.
HPLC is a
powerful method for identifying radiolabeled metabolites as molecular species
of various lipid classes. Chapters 1, 8, and 12 describe such identification
for metabolic studies in detail. Special techniques such as chiral (Chapter 7)
and silver-ion HPLC (Chapter 9), as well as separation of medically important
acyl lipids such as bioactive lipids (Chapters 6 and 15) and oxidized lipids
(Chapter13), are included in this book. Two chapters that are outside the realm
of acyl lipids are the HPLC of acyl CoA (Chapter16) and size-exclusion
chromatography of lipoproteins (Chapter 17). Supercritical-fluid chromatography
of lipids is also included as Chapter 18, because the technology is similar to
HPLC.
Many
internationally known lipid HPLC experts have contributed excellent chapters.
We are grateful to all 28 authors from 6 countries for sharing their expertise
in the various aspects of HPLC. Their contributions to science and to this book
are vital to meeting the objectives of this book. We also thank HNB Publishing
for their cooperation in bringing everything into print. As a result of these
efforts, this book will be useful to lipid scientists and other technologists
involved in academic, government, and industrial research requiring lipid
analysis, metabolic studies, and enzyme assays. Moreover, the book should serve
as a valuable resource in continuing HPLC method development.