HPLC of Acyl Lipids

Edited by Jiann-Tsyh Lin and Thomas A. McKeon

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April 2005
572 pp.
ISBN: 978-0-9828874-0-0

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High-performance liquid chromatography (HPLC) is a powerful method for lipid analysis. This book covers many practical and theoretical aspects of HPLC separation of acyl lipids. A book with a similar title, High-Performance Liquid Chromatography and Lipids: A Practical Guide, by William W. Christie, covering mainly acyl lipids, was published in 1987. Since that time, a great number of papers related to the HPLC of acyl lipids have been published, making an updated book devoted exclusively to the HPLC of acyl lipids long overdue. Since lipids in general are too broad to cover in one book, we have limited this book to fatty acids and fatty acid棯ntaining lipids as acyl lipids, and have excluded other lipids such as steroids, terpenoids, carotenoids and vitamin E (prenyl lipids).


This book's 18 chapters are aimed at the practical aspects of lipid separation. Since practical and theoretical aspects of lipid separation are interrelated, one chapter (Chapter 3) is devoted exclusively to the HPLC theory of acyl lipid separation. HPLC of lipid derivatives is not emphasized in this book, except in Chapter 1, covering fatty acid methyl esters; Chapter 2, devoted exclusively to fatty acid derivatives; and Chapter 7, covering chiral phase HPLC. LC-MS is a powerful tool for lipid analysis and has become more affordable and popular. Therefore, two chapters focus on LC-MS using atmospheric pressure chemical ionization (Chapter 10) and electrospray ionization (Chapter 11) for separation and identification. In addition, one chapter is devoted to the LC-MS-MS of bioactive lipids (Chapter 15), and many chapters also include some LC-MS.


HPLC is a powerful method for identifying radiolabeled metabolites as molecular species of various lipid classes. Chapters 1, 8, and 12 describe such identification for metabolic studies in detail. Special techniques such as chiral (Chapter 7) and silver-ion HPLC (Chapter 9), as well as separation of medically important acyl lipids such as bioactive lipids (Chapters 6 and 15) and oxidized lipids (Chapter13), are included in this book. Two chapters that are outside the realm of acyl lipids are the HPLC of acyl CoA (Chapter16) and size-exclusion chromatography of lipoproteins (Chapter 17). Supercritical-fluid chromatography of lipids is also included as Chapter 18, because the technology is similar to HPLC.


Many internationally known lipid HPLC experts have contributed excellent chapters. We are grateful to all 28 authors from 6 countries for sharing their expertise in the various aspects of HPLC. Their contributions to science and to this book are vital to meeting the objectives of this book. We also thank HNB Publishing for their cooperation in bringing everything into print. As a result of these efforts, this book will be useful to lipid scientists and other technologists involved in academic, government, and industrial research requiring lipid analysis, metabolic studies, and enzyme assays. Moreover, the book should serve as a valuable resource in continuing HPLC method development.

Contents | About the Editors

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