Contemporary Microscale Separation Technology

Chao Yan

 $99.75
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June 2013
342 pp.

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Illustrated:
ISBN: 978-0-9828874-2-4

 

Contents

Chapter 1

MULTIDIMENSIONAL LIQUID CHROMATOGRAPHY AND CAPILLARY ELECTROPHORESIS

ࠠࠠࠠࠠ1.1ࠠࠠ History

ࠠࠠࠠࠠ1.2ࠠࠠ Capillary Electrophoresis

ࠠࠠࠠࠠ1.2.1Instrumentation

ࠠࠠࠠࠠ1.2.2Capillaries

ࠠࠠࠠࠠ1.2.3Deactivation of capillary walls for CE of proteins

ࠠࠠࠠࠠ1.2.4Electromigration dispersion

ࠠࠠࠠࠠ1.2.5Injection

ࠠࠠࠠࠠ1.2.6Detection units

ࠠࠠࠠࠠ1.2.7Separation techniques

ࠠࠠࠠࠠ1.2.8Sample preconcentration

ࠠࠠࠠࠠ1.2.9Resolution enhancement with nonconventional electrolytes

ࠠࠠࠠࠠ1.3ࠠࠠ Capillary Zone Electrophoresis

ࠠࠠࠠࠠ1.4ࠠࠠ Micellar Electrokinetic Chromatography

ࠠࠠࠠࠠ1.4.1Introduction

ࠠࠠࠠࠠ1.4.2Fundamental characteristics

ࠠࠠࠠࠠ1.4.3Detection techniques

ࠠࠠࠠࠠ1.4.4On-line sample preconcentration

ࠠࠠࠠࠠ1.4.5Conclusions and prospects

ࠠࠠࠠࠠ1.5ࠠࠠ Capillary Isoelectric Focusing

ࠠࠠࠠࠠ1.5.1Introduction

ࠠࠠࠠࠠ1.5.2Modes

ࠠࠠࠠࠠ1.5.3Coating

ࠠࠠࠠࠠ1.5.4Detection

ࠠࠠࠠࠠ1.5.5Conclusion

ࠠࠠࠠࠠ1.6ࠠࠠ Capillary Gel Electrophoresis

ࠠࠠࠠࠠ1.6.1History

ࠠࠠࠠࠠ1.6.2Principle

ࠠࠠࠠࠠ1.6.3Sieving media

ࠠࠠࠠࠠ1.6.4Lab-on-a-chip

ࠠࠠࠠࠠ1.7ࠠࠠ Capillary Isotachophoresis

ࠠࠠࠠࠠ1.7.1Introduction

ࠠࠠࠠࠠ1.7.2Mobility and coating

ࠠࠠࠠࠠ1.7.3Detection

ࠠࠠࠠࠠ1.7.4Conclusion

ࠠࠠࠠࠠ1.8ࠠࠠ Applications

ࠠࠠࠠࠠ1.8.1Capillary zone electrophoresis

ࠠࠠࠠࠠ1.8.2Micellar electrokinetic capillary chromatography

ࠠࠠࠠࠠ1.8.3Capillary isoelectric focusing

ࠠࠠࠠࠠ1.8.4Capillary gel electrophoresis

ࠠࠠࠠࠠ1.8.5Capillary isotachophoresis

ࠠࠠࠠࠠReferences

 

Chapter 2

CAPILLARY ELECTROCHROMATOGRAPHY

ࠠࠠࠠࠠ2.1ࠠࠠ Capillary Electrochromatography

ࠠࠠࠠࠠ2.1.1Theory

ࠠࠠࠠࠠ2.1.2Instruments

ࠠࠠࠠࠠ2.1.3Columns

ࠠࠠࠠࠠ2.1.4Sample introduction and detection

ࠠࠠࠠࠠ2.2ࠠࠠ Pressurized Capillary Electrochromatography

ࠠࠠࠠࠠ2.2.1Fundamental aspects

ࠠࠠࠠࠠ2.2.2Instrumentation

ࠠࠠࠠࠠ2.2.3Columns used in pCEC

ࠠࠠࠠࠠ2.2.4Mass spectrometry coupling

ࠠࠠࠠࠠ2.2.5Applications

ࠠࠠࠠࠠReferences

 

Chapter 3

CAPILLARY LIQUID CHROMATOGRAPHY

ࠠࠠࠠࠠ3.1ࠠࠠ Introduction

ࠠࠠࠠࠠ3.2ࠠࠠ Equipment

ࠠࠠࠠࠠ3.2.1Injectors

ࠠࠠࠠࠠ3.2.2Solvent delivery systems

ࠠࠠࠠࠠ3.2.3Columns

ࠠࠠࠠࠠ3.2.4Tubing

ࠠࠠࠠࠠ3.2.5Detectors

ࠠࠠࠠࠠ3.3ࠠࠠ Stationary Phases

ࠠࠠࠠࠠ3.3.1Particles

ࠠࠠࠠࠠ3.3.2Monolithic columns

ࠠࠠࠠࠠ3.3.3Other stationary phases

ࠠࠠࠠࠠ3.4ࠠࠠ Applications

ࠠࠠࠠࠠ3.4.1Food and environment

ࠠࠠࠠࠠ3.4.2Drugs

ࠠࠠࠠࠠ3.4.3Proteins

ࠠࠠࠠࠠ3.4.4.Chiral separations

ࠠࠠࠠࠠReferences

 

Chapter 4

MICROFLUIDIC DEVICES FOR HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

ࠠࠠࠠࠠ4.1ࠠࠠ Introduction

ࠠࠠࠠࠠ4.2ࠠࠠ Open Tubular Columns

ࠠࠠࠠࠠ4.3ࠠࠠ Micromachined Pillar Array Columns

ࠠࠠࠠࠠ4.4ࠠࠠ Packed-Bed Columns

ࠠࠠࠠࠠ4.5ࠠࠠ Monolithic Columns

ࠠࠠࠠࠠ4.6ࠠࠠ HPLC-Chip/MS Platform

ࠠࠠࠠࠠ4.7ࠠࠠ Concluding Remarks

ࠠࠠࠠࠠReferences

 

Chapter 5

MULTIDIMENSIONAL LIQUID CHROMATOGRAPHY AND CAPILLARY ELECTROPHORESIS

ࠠࠠࠠࠠ5.1ࠠࠠ Introduction

ࠠࠠࠠࠠ5.2ࠠࠠ Capillary Liquid Chromatography‒Capillary Liquid Chromatographyࠠࠠࠠ࠼/span>

ࠠࠠࠠࠠ5.3ࠠࠠ Capillary Liquid Chromatography‒Capillary Electrophoresisࠠࠠࠠ

ࠠࠠࠠࠠ5.4ࠠࠠ Capillary Electrophoresis‒Capillary Liquid Chromatographyࠠࠠࠠࠠ

ࠠࠠࠠࠠ5.5ࠠࠠ Capillary Electrophoresis‒Capillary Electrophoresis

ࠠࠠࠠࠠ5.6ࠠࠠ Capillary Electrochromatography Pressurized Capillary Electrochromatography in Multidimensional Coupling

ࠠࠠࠠࠠ5.7ࠠࠠ Applications

ࠠࠠࠠࠠ5.7.1Metabolites

ࠠࠠࠠࠠ5.7.2Pharmaceuticals

ࠠࠠࠠࠠ5.7.3Cells and organelles

ࠠࠠࠠࠠ5.7.4Proteins

ࠠࠠࠠࠠ5.7.5Peptides

ࠠࠠࠠࠠ5.7.6Food

ࠠࠠࠠࠠ5.7.7Other Applications

ࠠࠠࠠࠠReferences

 

Abbreviations and Symbols

Index

About the Author | Preface

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